Advanced separation and imaging of living cells infrastructure core

Equipment:

Veritas Laser Capture Microdissection System

  • Laser-capture microdissection (LCM)  isolates subpopulations of tissue cells under direct microscopic visualization 
  • houses two lasers for the microdissection of individual cells or larger areas of tissue for proteomic and genomic analysis  
  • LCM technology captures the cells of interest directly, or can cut away unwanted cells to give enriched cell populations
  • offers both LCM and Laser Cutting
  • combines the advantages of confocal imaging with the versatility of conventional live cell microscopes  
  • "swept field" technology overcomes many of the limitations of traditional confocal systems, providing maximum flexibility of both speed and resolution

Services:

  • Multi-parameter analysis including (but not limited to): 
    • phenotyping
    • viability
    • apoptosis
    • DNA cell cycle and ploidy
    • cytokines
    • proliferation
    • fluorescent proteins
    • bead array
    • morphology
  • Data analysis
  • Consultation
  • Cell sorting
  • Experiment troubleshooting
  • Instrument training
  • Publication quality graphics
  • Lectures and workshops
  • Literature and protocol resources

Using the lab:

Contact lab supervisor Melissa Makris (540-231-4115), who is available to run samples, analyze samples, and assist in the development of research protocols utilizing this equipment.

Specific experiments are scheduled during regular business hours, and charges are assessed on an hourly basis.